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KMID : 0900919980220020195
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Korean journal of Animal Reproduction
1998 Volume.22 No. 2 p.195 ~ p.202
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Fertilization Process in Porcine Oocytes Following Intracytoplasmic Injection of Porcine, Human, Bovine or Mouse Spermatozoon
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Lee Hoon-Taek
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Abstract
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We demonstrated, for the first time, pronuclear formation and apposition in porcine ooc-ytes following intracytoplasmic injection of porcine, human, bovine and mouse spermatozoon. Microtubule organization and chromatin configuration were investigated in these oocytes during pronuclear apposition. Following intracytoplasmic injection of porcine spermatozoon, the microtubular aster was organized from the neck of spermatozoon, and filled the whole cytoplasm. This male derived microtubules appear to move both pronuclei to the center of oocytes. In contrast, following injection of spermatozoa from different species such as human, bovine and mouse, microtubules were organized from the cortex of the oocytes and concentrated to the pronuclei, which seems to move both male and female pronuclei to the center of oocyte. This organization is similar to what has been shown in the parthenogenetically activated por-cine oocytes. These results suggested that the porcine, human, bovine and mouse sperm chromatin can be formed pronucleus and apposited in the center of oocytes in the absence of male derived microtubule when they were injected into porcine oocytes.
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KEYWORD
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Porcine oocytes, ICSI, Fertilization, Centrosome
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